The PCR laboratory is exclusively dedicated to DNA amplification, in which we exponentially multiply specific genetic sequences, making them detectable and analysable. This technology is indispensable for the study of biodiversity, as it allows us to obtain a large amount of genetic material even from limited or degraded samples. PCR laboratory isolation is an indispensable precaution to guarantee the reliability of results, as even the slightest contamination could compromise the entire experiment. Laboratory isolation allows us to create a more controlled environment, minimising the risk of contamination by foreign DNA that may be incorporated even in aerosol form. At the same time, amplified DNA containing high amounts of nucleic acid copies must be isolated as much as possible in order to avoid contamination of other substrates. In the laboratory, purifications of the material are also carried out, which must then be assembled for genotyping, Real Time PCR and sequencing.
Relevant Instrumentation
- Thermal cyclers
- Microtubes centrifuge
- Ice crushing machine
- Freezers for DNA c DNA storage and tissue banks/filters for both animal and e DNA extraction. Storage of chemical material for carrying out amplifications
| Chief Scientific Officer | Mariella Baratti, Emiliano Mori |
|---|---|
| Team | Mariella Baratti, Emiliano Mori, Leonardo Ancillotto |
| Contacts | E-mail: mariella.baratti@cnr.it ; emiliano.mori@cnr.it ; leonardo.ancillotto@cnr.it |